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INDONESIA
BIOVALENTIA: Biological Research Journal
Published by Universitas Sriwijaya
ISSN : -     EISSN : 24771392     DOI : https://doi.org/10.24233/biov
Core Subject : Health, Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology
BIOVALENTIA: Biological Research Journal aims to review and publish the Biological research results from researchers. The range of articles which BIOVALENTIA: Biological Research Journal publishing is the research results in Biology science and applied Biology. Biology science is included Ecology, Biosystematics, Physiology, Developmental Biology, Microbiology, Genetics. Applied Biology is included Biotechnology, and the sciences derived from Biology science.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 10 Documents
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Search results for , issue "Vol. 9 No. 1 (2023)" : 10 Documents clear
ISOLATION AND PURITY DNA FROM LEAF STORAGE OF Lansium domesticum CORRESPOND FOR BARCODING ANALYSIS Nur Arifah; Laila Hanum; Sarno Sarno; Nita Aminasih; Singgih Tri Wardana; Hary Widjajanti
BIOVALENTIA: Biological Research Journal Vol. 9 No. 1 (2023)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.9.1.2023.251

Abstract

Lansium domesticum is one of tropical plant. Genetic studies of tropical plant species occurred very slowly due to some obstacles. Provided fresh sample for isolation step was one of impediment. Fresh sample was important conditions to avoid impure DNA template. A simple and rapid technique was needed to get a pure DNA from storage sample. The aims of this study is to see the purity of the DNA isolation results from Lansium domesti-cum mature leaf from South Sumatra that have been stored for 2 years and to test whether the DNA isolation results can be used for barcoding analysis by amplification using PCR method with rbcL primer. There are 11 sample of Lansium domesticum were taken from 8 districts in South Sumatra. The sample was the mature leaves of Lanisum domesticum. The leaves were stored in a freezer at -20oC for two years. There was one sam-ple stored within 2 weeks as a comparison. The results of the study showed the isolation of DNA from storage and mature leaves of Lansium domesticum used Plant Genomic DNA Kit DP305 had a good quantity for 7 sample. 3 sample had low purity and 1 sample contaminated by RNA.  Despite electrophoresis showed the isola-tion result not intact and compact, nevertheless the PCR results showed 11 samples could be amplified using rbcL primer. This result indicated the DNA isolation method suit for barcoding analysis using PCR method.
ANALYSIS OF PB CONTENT IN LEAVES OF COVERING PLANTS IN VARIOUS ROADS IN PALEMBANG CITY Hasdevi Hasdevi; Suheryanto Suheryanto; Susilawati Susilawati
BIOVALENTIA: Biological Research Journal Vol. 9 No. 1 (2023)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.9.1.2023.335

Abstract

Alternative Decree of the Director General of Oil and Gas No. 3674K/24/DJM/2006 dated March 17, 2006 concerning Standards and Quality (Specifications) of Gasoline-Type Fuel Oil Marketed Domestically where in the attachment to the Specification for Gasoline-Type Fuel Oil RON 91 and RON 95 (Pertamax) still contains Pb of 0.013 grams per liter. The exhaust gas produced by motorized vehicles using leaded fuel emits Pb into the environment and has the potential to be absorbed into the leaf tissue of road shade plants. The aim of this study was to examine the Pb content in the leaves and determine the effect of the Pb content in the ambient air on the Pb content in the leaves of various cover crops on Jalan Raya Palembang City. The research samples were leaves of mahogany, umbrella tree, burmese rosewood, bullet wood tree, and rain tree which were dominant and had similarities on Jl. Soekarno Hatta – Alamsyah Ratu Prawiranegara, Jl. Cabbage. H. Burlian, Jl. Jend. Sudirman, Jl. Jend. Basuki Rahmad, Jl. Jend. R. Soekanto, Jl. Jend. Ahmad Yani. Jl. governor HA. Bastari. Analysis of the Pb content in leaves and air using the AAS (Atomic Absorption Spectrophotometer) method. The results of the study found that the highest Pb content in the leaves of the Mahogany plant was on Jl. Jend. Basuki Rahmad 0.112 mg/kg while the lowest was on Jl. governor HA. Bastari <0.081 mg/kg. Pb content in umbrella tree plants in all roads < 0.081 mg/kg. The highest Pb content in burmese rosewood plants is on Jl. Jend. Basuki Rahmad 0.116 mg/kg while the lowest was on Jl. governor HA. Bastari <0.081 mg/kg. The highest Pb content in bullet wood tree Jl. Jend. Basuki Rahmad 0.099 mg/kg while the lowest is on Jl. governor HA. Bastari <0.081 mg/kg. The highest Pb content in rain tree is on Jl. Jend. Basuki Rahmad 0.089 mg/kg while the lowest is on Jl. governor HA. Bastari <0.081 mg/kg. The highest Pb content in ambient air is on Jl. Jend. Basuki Rahmad 0.06 ʯg/Nm3 while the lowest is on Jl. Jend. R. Soekarno. The Pb content in the ambient air affects the Pb content in the leaves.
EFFECTIVITY OF Lepironia articulate Retz (Domin.) AS AN ABSORBEND OF HEAVY METALS FE AND MN ACID MINE DRAINAGE Fira Hendri; Eddy Ibrahim; Hilda Zulkifli
BIOVALENTIA: Biological Research Journal Vol. 9 No. 1 (2023)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.9.1.2023.342

Abstract

Indonesia is one of the world's largest coal-producing countries. Most coal mining activities are conducted using the open-pit method. A classic problem caused by mining activities is acid mine drainage (AMD). Various conventional methods have been developed to increase the pH of the water and remove heavy metals such as iron (Fe) and manganese (Mn) in acid mine drainage, including chemical precipitation, coagulation, flocculation, microorganisms, adsorption, and passive treatment methods. In this study, a passive treatment method using constructed wetlands with phytoremediation techniques using L. articulate plants was employed. This study aims to analyze the effectiveness of the L. articulate plant in increasing the acidity of water and reducing the levels of Fe and Mn metals dissolved in acid mine drainage. The results of the initial sample analysis of acid mine drainage showed a pH of 2.8. The initial values of Fe met-al concentrations were 0.09 mg/L and Mn 8.10 mg/L. After the phytoremediation process, it was found that L. articulate plants were effective in increasing the pH value and reducing the concentrations of dissolved Fe and Mn metals in acid mine drainage within 20 days. The percentage reduction was 93.33% in the organic reactor and 55.56% in the inorganic reactor for Fe metal concentrations. For Mn metal reduction, the percentages were 38.02% in the organic reactor and 25.80% in the inorganic reactor.
COMPARISON OF PLANTING SHRUB PEPPER (Piper nigrum L.) FLOATING AND DRY LAND WITH MAGNESIUM FERTILIZER TREATMENT (Mg) Kartika Putri; Momon Sodik Imanuddin; Bakri Bakri
BIOVALENTIA: Biological Research Journal Vol. 9 No. 1 (2023)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.9.1.2023.370

Abstract

The cultivation of pepper plants is usually carried out on dry land, but at this time there is a lot of conversion of pepper plantations to other plantations and even housing. This study aimed to investigate the growth ratio of floating agricultural pepper and dry land, study the best Mg dosage for the growth of pepper with float-ing and dry land systems, andinvestigate the planting system on soil moisture content, pH and Mg content of soil and plants. The research was carried out from September 2020 to January 2021 in the gardens of the Soil Department, Faculty of Agriculture, Sriwijaya University, Indralaya. This research was conducted using the Split Plot Design method with 2 factors. As the main plot is the farming system, namely: S1 = Floating. S2 = On dry land (conventional). Subplots were Mg fertilizer with doses per polybag: Mg0 = No Mg fertilizer, Mg1 = 4g plant-1 , Mg2 = 8 g plant-1 , Mg3 = 12 g plant-1 , Mg4 = 16 g plant-1 . Observations were made on the increase in plant height, number of leaves, elemental content mg, soil water content, soil pH. The results showed that the planting system had an effect on plant height, number of leaves in the 11th week and root length, Mg doses did not affect the growth of pepper and soil. The soil water content of the floating system is higher than the conventional system, whereas the pH is higher in the conventional system than the floating system.
FIRST REPORT OF NEW WILT DISEASE ON Mangifera indica CAUSED BY Ceratocystis fimbriata IN INDONESIA Rahmat Pratama; Ahmad Muslim; Suwandi Suwandi
BIOVALENTIA: Biological Research Journal Vol. 9 No. 1 (2023)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.9.1.2023.377

Abstract

Ceratocystis wilt and canker disease has severely compromised the profitability of Mangifera indica plantations in the world. In 2022, wilt and sudden death were observed on Mangifera indica. Identification was performed by sequence analysis of the concatenated β-tubulin gene regions. Sequencing of the PCR product confirmed this pathogen was Ceratocystis fimbriata sensu stricto. This is the first report of C. fimbriata causing sudden death disease in M. indica in Indonesia.
ENDOPHYTIC TESTING OF Serratia marcescens strain NPKC3_2_21 AGAINST INPARA 3 RICE VARIETY Gunawan Sutio; Iskandar Iskandar; Lilik Tri Indriyati; Gunawan Djajakirana
BIOVALENTIA: Biological Research Journal Vol. 9 No. 1 (2023)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.9.1.2023.372

Abstract

Pest management is a crucial concern, especially when dealing with insect pests that can cause extensive damage to agricultural crops and the economy. One such pest is the rice stem borer (Scirpophaga innotata), which infests rice stems and poses a significant threat. To combat these pests, various microbial agents have been developed for effective pest control. Among these, the endophytic microbe in biological plant protection plays a key role in the program of sustainable pest management. An endophytic bacterium, Serratia marcescens strain NPKC3_2_21, has been identified as a key player in sustainable pest management, particularly in rice crops. In this study, we aimed to investigate the endophytic characteristics of S. marcescens strain NPKC3_2_21 in the swamp rice plant of the Inpara 3 variety of swamp rice plants. To establish its roles. Marcescens as an endophytic bacterium in rice plants, specifically the Inpara 3 variety, we conducted tests by inoculating bacteria on the plant tissue of rice plants that have been sterilized rice plant tissues with the bacteria. We assessed the presence of S. marcescens strain NPKC3_2_21 in plant tissues by applying isolates to the surface of rock wool, which supported the growth of wet rice plants aged ten or over ten days or older after planting. Samples were collected from the underside of the stem, the bottom of the leaves, and the roots on days 2, 7, and 14 after the application of isolates to the rock wool. The samples were then washed in 70% alcohol and 4% chlorox for 30 seconds and subsequently isolated on Luria Bertani (LB) agar media. Furthermore, we conducted tests to determine the ligninolytic, cellulolytic, and proteolytic activities of S. marcescens, which helped elucidate its endophytic ability. Based on the result, the endophytic capabilities. Based on the results, we found that S. marcescens strain NPKC3_2_21 exhibited endophytic characteristics solely in the stem tissue of the Inpara 3 rice variety. However, we did not observe its presence in the root and leaf tissues.
EXPENDITURE ON OIL PALM FARMERS’ HOUSEHOLDS IN BAYUNG LENCIR SUBDISTRICT MUSI BANYUASIN REGENCY Khosy Khoirunnisa; Riswani Riswani; Lifianthi Lifianthi
BIOVALENTIA: Biological Research Journal Vol. 9 No. 1 (2023)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.9.1.2023.379

Abstract

The profitable business potential of palm oil entices the community and the Indonesian Government to develop it. It makes palm oil farming as a major source of farmers’ livelihood. However. oil palm plantations confront a number of challenges. including unstable prices and production. This will affect the household income obtained by farmers. The aim of this research is to examine the characteristics of oil palm farming. such as production costs. revenues and income as well as the proportion of household expenditure devoted to food and non-food consumption. This study was carried out in Bayung Lencir District. Musi Banyuasin Regency. South Sumatra Province. The disproportionate random sampling was used to acquire data from 90 respondents who are independent oil palm farmers. The depth interview method was used to acquired data. According to the findings. oil palm farmers’ income was IDR. 122,143,547/cultivation area/year or IDR. 10,178,629/cultivation area/month. The annual household income of oil palm farmers derived form on farm income other than oil palm, off farm income and non-farm income. The proportion of household expenditure for food consumption was 26.30%. while the proportion for non-food consumption was 73.70% and indicating that oil palm farming households are classified as prosperous since there is IDR. 1.015.871/month to be saved.
PROPAGATION OF Cnidoscolus aconitifolius USING STEM CUTTINGS AT DIFFERENT MATURITY STAGES AND GROWING MEDIA Fitra Gustiar Mashadi; Benyamin Lakitan; Dedik Budianta; Zaidan P Negara; M. Umar Harun; Susilawati Susilawati; Straiker Ali Muda
BIOVALENTIA: Biological Research Journal Vol. 9 No. 1 (2023)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.9.1.2023.382

Abstract

Chaya leaves (Cnidoscolus aconitifolius (Mill.) I.M. Johnst.) are leafy vegetables that contain many vitamins and minerals. Information about the intensification of chaya cultivation is still rare. Chaya propagated by stem cuttings. The maturity level of stem cuttings and planting media were expected to affect the success of plant propagation. This study aims to determine the maturity level of cuttings material that can grow well and deter-mine the type of media that can support the growth of chaya propagation. This research has been carried out from July to October 2022 in an off-campus experimental outdoor facility located in Inderalaya, South Suma-tra. This study was conducted using a randomized block design with 2 factors. The first factor was the level of maturity of stem cuttings material (upper, middle, and basal), and the second factor was the type of growing medium (control, chicken manure, and cow manure). The results showed that the use of cuttings from the mid-dle and basal has a better percentage of growth ability than the upper cuttings. Chaya plants used basal stem cuttings exhibited slower shoot grow but produced higher number of leaves, greater leaf area, canopy area, and biomass. The use of cow manure in a mixture of growing medium gave the best results on growth of chaya plants.
ARTIFICIAL SHADE ADAPTATION AND POPULATION DENSITY ON SWISS CHARD (Beta vulgaris subsp. Cicla (L) W.D.J Koch) IN URBAN AR-EA Rofiqoh Ria; Benyamin Lakitan; Firdaus Sulaiman; Yakup Yakup; Zaidan P Negara; Susilawati Susilawati
BIOVALENTIA: Biological Research Journal Vol. 9 No. 1 (2023)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.9.1.2023.384

Abstract

Swiss chard is a leafy vegetable that is high in nutrients, vitamins, minerals, protein, and antioxidants that are beneficial to human health. Swiss chard. Swiss chard is generally cultivated in the Mediterranean climate, grows well in full sunlight, air temperatures 14-21oC, and can still survive at temperatures close to light frost. The aim of this study to evaluate efficient plant densi-ties in urban limited land area and looking at the response of Swiss chard plant to reduc-ing the intensity of sunligt of 45%, 55% and 80%.in urban tropic area. This research was used 4 different artificial shading i.e 0% (control, shade 45%, shade 55% and shade 80%. Microclimate was measured per each shade for 14 days i.e., air temperatur, soil temperature, air humidity, and soil moisture using a data logger meter. Shade with intensity 80% is proven to reduce air temperate and soil temperature, but also inhibits the growth and development of Swiss chard plants. Population 1plant/pot gives the best growth and yield of Swiss chard per plant, 3 plants/pot increased total fresh weight per cultivation area, thereby maximize the use of limited urban land
COMMUNITY-BASED RAPID BIODIVERSITY ASSESSMENT: A PRELIMINARY STUDY IDENTIFYING POTENTIAL NATURAL RESOURCES IN KOTA AGUNG UTARA SOCIAL FORESTRY, TANGGAMUS Elly Lestari Rustiati; I Gede Swibawa; Jani Master; Alvin Wiwiet Susanto; Rendi Hasarudin; Joko Suprianto; Supriyadi Supriyadi; Edi Edi; Tugino Tugino; Andi Aryandinata; Dian Neli Pratiwi; Indra Kurniawan; Okta Saputra; Intan Diani Fardinatri; Mariman Mariman
BIOVALENTIA: Biological Research Journal Vol. 9 No. 1 (2023)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.9.1.2023.389

Abstract

Community-based rapid biodiversity assessment has been done initially with Gapoktanhut Lestari Sejahtera, a group of coffee farmers practises social forestry in protected forest, Sedayu, Semaka, Tanggamus, under supervision of KPH Kotaagung Utara, Tanggamus.   Its aim is to identify potential natural resources in Kotaagung Utara protected forest, Tanggamus, under Kaidenren Nature Conservation Fund, in collaboration with University of Lampung and Rainforest Alliance program. Direct survey and observation were done, kobotoolbox was applied to collect the biodiversity information, the direct and indirect data of plants, animals as well as landscapes.  Of 480 data input has been done by transect line from November, 2021 to February, 2022 covering 15 observed areas.  The data consists of direct and indirect data of plants, animals as well as landscapes. The finding done is by local names included plants (n=78) mostly understory plant species, orchids, and ferns, small animals (n=40) include amphibians, reptiles, birds, and arthropods, indirect signs (n=11) of food remains, footprints, voices, scraps, wallowing ponds, scratches, nests, insects body remains, eggs and animals’ trail, some fungi, and landscapes. The findings shows that local community is potential citizen research resources, supporting social forestry practice and the initial building of Kota Agung Utara Forest Management Unit database

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